Multimodal fast imaging

Non mechanical scanner
Fullfield scan up to 40Hz
Roi scan up to KHz
Multiplan scan up to 20Hz

 

Ultrafast random access

Unique speed recording
Point of interest up to 100KHZz/POIs
Ultrafast local volumetric excitation up to 20KHz/VOIs
Validated voltage indicator recording

Evolutive design

Customized configuration
Upgradable and extendable
Up to 2 scanner channels

High-end hardware

highest performances and usability
Ghz high speed electronic
Photon counting
Giga sample analog acquisition
Ultra precise synchronisation

 

 

 

Fast and Silent Imaging at tunable speed

AODs are fast pointers but also fast scanners (patented) which can surpass resonant galvanometric mirrors, while remaining completely silent.
The inertia-free angular scanning yields utmost linearity, tunability and repeatability, as compared to the sinusoidal scanning behavior of resonant galvos.

Multiple Z planes and Multiple Regions Of Interest (ROIs)
Resonant galvanometric mirrors can only address a set of adjacent lines of the same size and in the same Z-plane, limiting your experimental capabilities.
Our AOD system can scan seamlessly multiple ROIs at resonant speed as well as multiple Z planes. Flight time between any ROIs or Z-planes is 10 µs.

Tunable dwell time (50ns/pixel → ∞/pixel)

 Quiet scanning for unbiased behaviour experiments

Freely selectable Multi ROIs up to maximum speed

Multi-plane imaging at different Z with no moving parts

 


Beam conditioner
Expansion ratio : variable 3-9x with Integrated alignment diagnotics

Scan paths
First acousto optic scanning
One-click wavelength tunability over 200 nm/scanning
Second acousto optic scanning
One-click wavelength tunability over 200 nm/scanning
Used separate or simultaneous (stimulation)

Scan FOV
Typical :
350 µm x 350 µm with 25x objective
500 µm x 500 µm with 16x objective
Can be modified on request , depending on beam diameter at objective backaperture

Recording capabilities
Scanning imaging speed : adjustable up to 10MHz ( 100ns/pixel), 38fps (512x512)
Multi ROIs to optimize dwell time, frame rate and sensitivity
Random access speed : 100kHz / POIs (Points Of Interest)
Ultrafast LOcal Volume Excitation (ULOVE) : 20 kHz / VOI (Volume Of Interest)

Detector
Epi Path :
High efficiency liquid guide design (5mm)
Large collection Angle
32 mm filter and XL dichroic
Protected shutter for intense illumination
1 or 2 Hamamatsu GaAsP 40uA detectors with/without gated
Trans path (in Vitro frame) :
1 Laser scanning DODT contrast
High efficiency liquid guide design (5mm)
Large collection Angle
32 mm filter and XL dichroic
1 or 2 Hamamatsu GaAsP 40uA detectors with/without gated

Microscope Frame

Software
Multi scan channel interface
Automatic 3D stabilization of episodic acquisitions (during the inter-episode dwell time)
External triggers, electrophysiology synchronization
Hardware control (microscope, stages, laser, camera)
Data acquisition and saving, easy data post-processing on open image and data formats
Embedded data visualizer

Electronic control
Future-ready opto-mechanics designed for integration of new features
High-speed programmable electronics supporting next generation sensors and signal processing
High efficiency proprietary Photon counting or analog integration
Additionnal Ni card for external in out on demand (software control)