Multimodal fast imaging
Non mechanical scanner
Fullfield scan up to 40Hz
Roi scan up to KHz
Multiplan scan up to 20Hz
Ultrafast random access
Unique speed recording
Point of interest up to 100KHZz/POIs
Ultrafast local volumetric excitation up to 20KHz/VOIs
Validated voltage indicator recording
Evolutive design
Customized configuration
Upgradable and extendable
Up to 2 scanner channels
High-end hardware
highest performances and usability
Ghz high speed electronic
Photon counting
Giga sample analog acquisition
Ultra precise synchronisation
Fast and Silent Imaging at tunable speed
AODs are fast pointers but also fast scanners (patented) which can surpass resonant galvanometric mirrors, while remaining completely silent.
The inertia-free angular scanning yields utmost linearity, tunability and repeatability, as compared to the sinusoidal scanning behavior of resonant galvos.
Tunable dwell time (50ns/pixel → ∞/pixel)
Quiet scanning for unbiased behaviour experiments
Freely selectable Multi ROIs up to maximum speed
Multi-plane imaging at different Z with no moving parts
Why scan a whole image when you can point to your cells of interest and spend all the frame time collecting useful signal?
Record from 300 cells at 300 Hz and resolve fluorescence transients temporally with enhanced signal to noise ratio. This can be done seamlessly from an image either manually or through automatic object recognition.
We have recently demonstrated that AODs can be used as wavefront shapers. By generating chirped patterns of acoustic frequencies, light can be deflected to different directions at each point of the laser beam profile, distorting the wavefront in the desired way. Wavefront shaping opens a new realm of capabilities, from defocalisation to holographic patterning. Acousto-optic wavefront shaping combines the best of three worlds: Random Access, Fast local 3D scanning and holographic patterning to perform Ultrafast Local Volume Excitation. U gonna LOVE it.
Holographic volume expansion
Mitigated in vivo movement artifacts
Enhanced optogenetic stimulation efficiency
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Evolutive design
The AODscope platform features a future-ready opto-mechanics design, imagined for easy customization and integration of new features. We want our customers to have an instrument with an unlimited lifetime. In vitro or in vivo microscope frame Our team works on the upcoming breakthroughs : adaptive optics, tunable lenses, temporal focusing, multiplexing, new detectors. All these developments will be compatible with the platforms.
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High-end hardware
Hardware control: Ultra-high precision RF acousto-optic generation and low noise signal acquisition by proprietary electronics design High-speed programmable electronics supporting next generation sensors and signal processing Photon counting and photocurrent integration External synchronization and trigger I/O
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Karthala imaging software
User friendly software Multi scan channel interface Automatic 3D stabilization of episodic acquisitions (during the inter-episode dwell time) External triggers, electrophysiology synchronization Hardware control (microscope, stages, camera) Data acquisition and automatic saving, easy data post-processing on open image and data formats Embedded data visualizer
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brain slice, interneuron td tomato in red, microglia eGFP in green
laser contrast and purkinje cell loaded with alexa594
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Zebrafish Gcamp 6s+ SHG
Zebrafish iGluSnFR ( CSHL, Imaging Structure & Function in the Nervous System 2019)
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cerebellum ASAP3b + JR Geco
culture neuron ASAP3 + laser contrast
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visual cortex td tomato
Zebra fish SHG